Kick-off is at 18h00 CAT.
The game is scheduled to take place at the Stade du 28 Septembre in the Guinean city of Conakry.
Horoya advanced to the group stage after eliminating Libyan club Al Nasr in the first round tie towards the end of last month.
The reigning Guinean Championnat National champions secured a 6-5 victory on aggregate – winning 6-2 at home in the second-leg clash.
Bolaji Simeon Sakin, who scored twice against Al Nasr, is the dangerman for the HAC.
Meanwhile, Esperance are the defending champions and they automatically qualified for the group stage.
The Tunisian Ligue Professionnelle 1 champions clinched last year’s title after defeating Egyptian football powerhouse Al Ahly in the final.
Esperance’s Algerian striker Youcef Belaili is a key player and he will look to inspire the team to victory.
Ethiopian referee Bamlak Tessema Weyesa will be in charge of the match between Horoya and Esperance.